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Product Info Summary
| SKU: | EKC1894 |
|---|---|
| Size: | 1 kit, containing one 96-well plate and all necessary reagents |
| Reactive Species: | Human, Mouse |
| Application: | ELISA |
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Product info
Product Name
TF3B Colorimetric Cell-Based ELISA
SKU/Catalog Number
EKC1894
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The TF3B Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor TF3B protein expression profile in cells. The kit can be used for measuring the relative amounts of TF3B in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on TF3B.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
TF3B Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1894)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1894 is reactive to BRF1 in Human, Mouse samples
Application Guarantee
EKC1894 is guaranteed for ELISA in Human, Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-BRF1 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 6ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
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Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from HeLa/Jurkat cells
Protein Target Info & Infographic
Gene/Protein Information For BRF1 (Source: Uniprot.Org, NCBI)
Gene Name
BRF1
Full Name
Transcription factor IIIB 90 kDa subunit
Weight
73.84kDa
Superfamily
TFIIB family
Alternative Names
B-related factor 1; BRF1 homolog, subunit of RNA polymerase III transcription initiation factorIIIB (S. cerevisiae); BRF-1; BRFFLJ42674; general transcription factor IIIB, 90kD subunit; GTF3BTAFIII90; hBRFMGC105048; hTFIIIB90; TAF3B2; TAF3CB - related factor 1; TATA box binding protein (TBP)-associated factor 3C; TATA box binding protein (TBP)-associated factor, RNA polymerase III, GTF3Bsubunit 2; TATA box binding protein (TBP)-associated factor, RNA polymerase III, subunit 2; TATA box-binding protein-associated factor, RNA polymerase III, subunit 2; TBP - associated factor, RNA polymerase III BRF1 BRF, BRF-1, CFDS, GTF3B, HEL-S-76p, TAF3B2, TAF3C, TAFIII90, TF3B90, TFIIIB90, hBRF BRF1 RNA polymerase III transcription initiation factor subunit transcription factor IIIB 90 kDa subunit|B - related factor 1|BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB|BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit|TATA box binding protein (TBP)-associated factor 3C|TATA box binding protein (TBP)-associated factor, RNA polymerase III, GTF3B subunit 2|TBP - associated factor, RNA polymerase III, 90kD|epididymis secretory sperm binding protein Li 76p|general transcription factor IIIB, 90kD subunit
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on BRF1, check out the BRF1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for BRF1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For TF3B Colorimetric Cell-Based ELISA (EKC1894)
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